[Methods-and-reagents] 2nd strand with klenow
Tim_HART at nea.gov.sg
Tue May 31 22:33:49 EST 2005
We have an RNA archive which I want to keep for as long as possible. I am
trying to at least get a cDNA copy (with random primers) of the important
RNA, so that we don't have to defrost and resuspend the RNA every time we
want to use it. But I have been told that ssDNA isn't as stable as dsDNA.
I am playing around with using Klenow to make the cDNA into dsDNA. Has
anyone experience in this? Has anyone got a simple protocol for second
strand synthesis? Is it possible to combine 1st and second strand
synthesis into one reaction?
Thanks a lot
Tim Hart, PhD
Environmental Health Institute
The National Environment Agency
DID +65 67719113
Fax +65 67778029
Our Environment -- We Care
This message may contain confidential information under the purview of the
Official Secrets Act. Unauthorized communication or disclosure of such
information is an offence under the Official Secrets Act. If you are not
the intended recipient of this message, please notify the sender and
delete it. Do not retain it or disclose the contents to any person as it
may be an offence under the Official Secrets Act.
Find out more about how we can work together to help Singapore keep a
clean and healthy environment at http://www.nea.gov.sg
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Methods