Protein binding in low salt buffers

Sanjay Vijendran sv213 at cam.ac.uk
Mon Nov 7 04:27:46 EST 2005


Hi all,

 Could anyone tell me whether it is possible to do protein binding
assays with the reagents in a buffer with 10e-4 M buffer solution?
Typically most buffers are in the 10mM range, but I need to do some
binding with much lower concentrations of buffer solution. Will the
binding kinetics or protein activity be affected by the low salt in
the buffer like it is with DNA hybridizations?

Thanks,
Sanjay



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