sharp.tool at bigpond.net.au
Mon Nov 14 05:40:32 EST 2005
Normally a 0.5 - 1.0 ng/ul of purified plasmid DNA is used for particle bombardment.
QUESTION: Does anyone use a commercial miniprep kit to purify plasmid DNA from an overnight culture to achieve this concentration? I found that the yield is very low, and no way of getting this concentration unless I precipitate the DNA and resuspend it in a smaller volume.
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