Particle bombardment

Duncan Clark blackhole at abuse.plus.com
Tue Nov 15 08:12:08 EST 2005


Historians believe that in newspost 
<AQZdf.17157$Hj2.2815 at news-server.bigpond.net.au> on Mon, 14 Nov 2005, 
Sharp Tool <sharp.tool at bigpond.net.au> penned the following literary 
masterpiece:
>Hi,
> 
>Normally a 0.5 - 1.0 ng/ul of purified plasmid DNA is used for particle 
>bombardment.
> 
>QUESTION: Does anyone use a commercial miniprep kit to purify plasmid 
>DNA from an overnight culture to achieve this concentration? I found 
>that the yield is very low, and no way of getting this concentration 
>unless I precipitate the DNA and resuspend it in a smaller volume.

What is the vector and in particular it's ori?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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