Coupled prokaryotic transcription/translation system

Alex ayboro at yahoo.com
Wed Nov 16 11:45:14 EST 2005


Hi all,
We are looking for a system that allows HTP generation of mkg amounts
of protein from linear PCR products. It seems that coupled prokaryotic
T7 driven is a way to go. Any suggestions or ideas? What would be the
most critical parameters? What yield to expect? Are there any
preferences among the commercial products?
Any help or advice will be greatly appreciated.
Thanks,
Alex



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