Coupled prokaryotic transcription/translation system

Duncan Clark blackhole at abuse.plus.com
Thu Nov 17 06:30:45 EST 2005


Historians believe that in newspost 
<1132159514.159552.279750 at g47g2000cwa.googlegroups.com> on Wed, 16 Nov 
2005, Alex <ayboro at yahoo.com> penned the following literary masterpiece:
>We are looking for a system that allows HTP generation of mkg amounts
>of protein from linear PCR products. It seems that coupled prokaryotic
>T7 driven is a way to go. Any suggestions or ideas? What would be the
>most critical parameters? What yield to expect? Are there any
>preferences among the commercial products?
>Any help or advice will be greatly appreciated.

mkg?

mg's or ug's?

 From what I have heard (never used it) the highest production seems to 
be using the Roche system. Maybe a look at how they do wit will give you 
some ideas of what to look for.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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