Particle bombardment

Oliver Berkowitz oliver.berkowitz at anu.edu.au
Wed Nov 16 18:34:57 EST 2005


Hi,

actually my handbook doesn't, ok it says 20ug instead of the 25ug I get. Maybe my UV spec overestimates a bit, but still it's not a lot less. Are you sure you are using the right handbook/kit ? And what kind of vector/ori are you working with ?

Cheers
Oliver

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Dr. Oliver Berkowitz 
The Australian National University 
Research School of Biological Sciences 
Environmental Biology Group 
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  "Sharp Tool" <sharp.tool at bigpond.net.au> wrote in message news:61Cef.18969$Hj2.11487 at news-server.bigpond.net.au...
  Not sure how you are able to achieve 0.5ug/ul with QIAPreps.
  The handbook indicates alot less than that.

  Cheers
  Sharp Tool

    Hi,

    I always used QIApreps (usually 0.5ug/ul) for my bombardments and that worked every time... You might just increase the volume of DNA you put to particle solution, doesn't matter much how you ppt the DNA on the particles.

    Cheers
    Oliver
    -- 
    _______________________________

    Dr. Oliver Berkowitz 
    The Australian National University 
    Research School of Biological Sciences 
    Environmental Biology Group 
    GPO Box 475 
    Canberra ACT 0200 
    Australia 

    P:  +61 - (0)2 - 61254549
    F:  +61 - (0)2 - 61254919
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