ladder pattern in gDNA extractions
pjie2 at cam.ac.uk
Tue Nov 29 17:20:53 EST 2005
mwcrepeau at hawaii.rr.com wrote:
>My questions are these:
>1. Have others seen this ladder pattern in their gDNA extractions?
>2. Does my "Hewish and Burgoyne redux" interpretation seem plausible?
>3. Could endogenous enzymatic activity be at fault?
>4. Can anyone offer suggestions on how to prevent the DNA degradation?
>For the coral work the cells were laboriously collected and purified by
>density-gradient centrifugation and flow cytometry in order to
>eliminate intracellular algal symbionts present in the species.
Isn't it simply apoptotic DNA laddering? The laborious pretreatment
could easily be leading to cell death.
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