ladder pattern in gDNA extractions

Peter Ellis pjie2 at
Tue Nov 29 17:20:53 EST 2005

mwcrepeau at wrote:
>My questions are these:
>1.  Have others seen this ladder pattern in their gDNA extractions?
>2.  Does my "Hewish and Burgoyne redux" interpretation seem plausible?
>3.  Could endogenous enzymatic activity be at fault?
>4.  Can anyone offer suggestions on how to prevent the DNA degradation?
>For the coral work the cells were laboriously collected and purified by
>density-gradient centrifugation and flow cytometry in order to
>eliminate intracellular algal symbionts present in the species.  

Isn't it simply apoptotic DNA laddering?  The laborious pretreatment 
could easily be leading to cell death.


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