ladder pattern in gDNA extractions

Peter Ellis pjie2 at cam.ac.uk
Tue Nov 29 17:20:53 EST 2005


mwcrepeau at hawaii.rr.com wrote:
>
>My questions are these:
>
>1.  Have others seen this ladder pattern in their gDNA extractions?
>2.  Does my "Hewish and Burgoyne redux" interpretation seem plausible?
>3.  Could endogenous enzymatic activity be at fault?
>4.  Can anyone offer suggestions on how to prevent the DNA degradation?
>
>For the coral work the cells were laboriously collected and purified by
>density-gradient centrifugation and flow cytometry in order to
>eliminate intracellular algal symbionts present in the species.  

Isn't it simply apoptotic DNA laddering?  The laborious pretreatment 
could easily be leading to cell death.

Peter


More information about the Methods mailing list