ladder pattern in gDNA extractions
NoReply at email.com
Tue Nov 29 18:15:40 EST 2005
In article <MPG.1df6e4a3a10de900989d0c at news.individual.net>,
Peter Ellis <pjie2 at cam.ac.uk> wrote:
> mwcrepeau at hawaii.rr.com wrote:
> >My questions are these:
> >1. Have others seen this ladder pattern in their gDNA extractions?
> >2. Does my "Hewish and Burgoyne redux" interpretation seem plausible?
> >3. Could endogenous enzymatic activity be at fault?
> >4. Can anyone offer suggestions on how to prevent the DNA degradation?
> >For the coral work the cells were laboriously collected and purified by
> >density-gradient centrifugation and flow cytometry in order to
> >eliminate intracellular algal symbionts present in the species.
> Isn't it simply apoptotic DNA laddering? The laborious pretreatment
> could easily be leading to cell death.
That's exactly what it is; and quite a nice demonstration of it!
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