antibody solution in milk gettin sour fast

MMu brilhasti at gmx.net
Tue Oct 4 05:20:36 EST 2005


"DK" <dk at no.email.thankstospam.net> schrieb im Newsbeitrag 
news:dhjm4k$1ff$1 at news.doit.wisc.edu...
> In article <433d32e8$0$11352$3b214f66 at usenet.univie.ac.at>, "MMu" 
> <brilhasti at gmx.net> wrote:
>>Our primary antibody solutions are getting sour very fast even though we 
>>use
>>natrium azide and store at 4°C at all times. Incubation is done at 4°C
>>overnight as well.
>>
>>1) is there a way to rescue an antibody solution after the milk has gone
>>sour?
>>2) is there a way to preserve the antibody solution any better then
>>described?
>
> I always dilute primary Ab into 3% BSA in TBST containing 4 mM
> azide. This way, it can be stored and reused literally for years.
> Secondary is always in milk - cleaner and cheaper and secondary
> are usually not worth reusing.
>
> DK

Thanks, I will try that! Funny that I never thought of using BSA instead... 




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