SDS and acetate in DNA isolation

[Berislav Lisnic]

Hello everyone,
 
Could anyone please provide explanations to the following questions (I've been doing quite an amount of minipreps, and i would really like to know the answers)...
 
1. How SDS disrupts bacterial cell wall/membrane?
2. Why is acetate used for precipitation of proteins in DNA isolation, and what is the apparent mechanism of  it's action?
 
Thanks in advance....

		
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