SDS and acetate in DNA isolation

Berislav Lisnic berislavlisnic at yahoo.com
Tue Oct 11 04:14:38 EST 2005


Hello everyone,
 
Could anyone please provide explanations to the following questions (I've been doing quite an amount of minipreps, and i would really like to know the answers)...
 
1. How SDS disrupts bacterial cell wall/membrane?
2. Why is acetate used for precipitation of proteins in DNA isolation, and what is the apparent mechanism of  it's action?
 
Thanks in advance....

		
---------------------------------
 Yahoo! Music Unlimited - Access over 1 million songs. Try it free.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/methods/attachments/20051011/dde568a5/attachment.html


More information about the Methods mailing list