SDS and acetate in DNA isolation

Berislav Lisnic berislavlisnic at
Tue Oct 11 04:14:38 EST 2005

Hello everyone,
Could anyone please provide explanations to the following questions (I've been doing quite an amount of minipreps, and i would really like to know the answers)...
1. How SDS disrupts bacterial cell wall/membrane?
2. Why is acetate used for precipitation of proteins in DNA isolation, and what is the apparent mechanism of  it's action?
Thanks in advance....

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