FACS on stromal cells ex vivo

Ian A. York iayork at panix.com
Tue Oct 18 14:33:53 EST 2005


In article <1129663500.476748.23050 at g43g2000cwa.googlegroups.com>,
Nick Theodorakis <nick_theodorakis at hotmail.com> wrote:
>
>Have you tried to get rid of the RBCs by Ficoll gradient? They are
>pretty dense and pellet through Ficoll (as also do PMNs), whereas most
>mononuclear cells will not.

Haven't tried that.  Do you have a protocol and/or reference handy?  

Ian 

-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England


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