crosslinking

Bean Long ben.long at yourfinger.anu.edu.au
Thu Sep 1 18:26:52 EST 2005


I have just started using the reversible cross-linker DSP.  It is useful for
cross-liniking in-vivo as it is relatively hydrophobic and can cross
membranes.  Nonetheless, I am using it in vitro after dissolution in DMSO
and it works fine.  There is a water soluble cousion (DTSSP) which can be
used in a similar way.  Both form cross-links which can be reversed under
reducing conditions.  They are both part of the huge Pierce stable of
cross-linkers for which Pierce has a little book to help you pick the one
you want.  I recommend you check out their website.

I have also used formaldehyde according to Vasilescu et al. (2004)
Proteomics 4:3845-3854. but found it a little too non-specific for my
particular application.  Formaldehyde cross-linking can also be reversible
under conditions of high temp.

Best of luck

-- 
Bean

Remove "yourfinger" before replying
"as" <nospam at nospam.com> wrote in message
news:df6biu$6uj$1 at news.net.uni-c.dk...
> I want to study whether my protein on the surface of a cell is present as
a
> homodimer! I will do this by crosslinking the proteins and running a wb.
> However there are so many crosslinking reagents, and I am not sure which
to
> choose. Does anyone have any suggestions.
> Thanks
> AS
>
>




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