crosslinking

Bean Long ben.long at yourfinger.anu.edu.au
Thu Sep 8 18:49:38 EST 2005


You may also find this reference from Pierce useful
http://www.piercenet.com/files/Cross-LinkingTechHB.pdf

-- 
Bean

Remove "yourfinger" before replying
"Bean Long" <ben.long at yourfinger.anu.edu.au> wrote in message
news:43178e3e$1 at clarion.carno.net.au...
> I have just started using the reversible cross-linker DSP.  It is useful
for
> cross-liniking in-vivo as it is relatively hydrophobic and can cross
> membranes.  Nonetheless, I am using it in vitro after dissolution in DMSO
> and it works fine.  There is a water soluble cousion (DTSSP) which can be
> used in a similar way.  Both form cross-links which can be reversed under
> reducing conditions.  They are both part of the huge Pierce stable of
> cross-linkers for which Pierce has a little book to help you pick the one
> you want.  I recommend you check out their website.
>
> I have also used formaldehyde according to Vasilescu et al. (2004)
> Proteomics 4:3845-3854. but found it a little too non-specific for my
> particular application.  Formaldehyde cross-linking can also be reversible
> under conditions of high temp.
>
> Best of luck
>
> -- 
> Bean
>
> Remove "yourfinger" before replying
> "as" <nospam at nospam.com> wrote in message
> news:df6biu$6uj$1 at news.net.uni-c.dk...
> > I want to study whether my protein on the surface of a cell is present
as
> a
> > homodimer! I will do this by crosslinking the proteins and running a wb.
> > However there are so many crosslinking reagents, and I am not sure which
> to
> > choose. Does anyone have any suggestions.
> > Thanks
> > AS
> >
> >
>
>




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