ethidium bromide vapour?

Jayakumar, R R.Jayakumar at
Sun Sep 11 12:01:44 EST 2005

The technique you describe is the preparatin of prestained agarose gels.  But actually it is never added to nearly boiling agarose.  The agarose is cooled till you can tolerate the temperature for 10 sec on your palm (should be about 50-60C).  Add ethidium bromde stock (10 mg/ml) 1 ul for 50 ml of solution.  Pour them into casting trays and allow to solidify.  You can add even less if the gel fluoresces badly.  Remember you cant destain prestained gels very easily.  
   Remember to dispose off the gel and all solutions that comes into contact with it carefully according to biohazard disposal procedures of your institute.
   I hope that helps. 

-----Original Message-----
From: methods-bounces at
[mailto:methods-bounces at]On Behalf Of jg374 at
Sent: Saturday, September 10, 2005 6:01 PM
To: methods at
Subject: ethidium bromide vapour?

A couple of people in our lab add ethidium bromide solution to hot agarose 
(i.e. nearly boiling), and claim that ethidium bromide cannot escape as a 
gas so there is no danger. This runs contrary to everything else I have 
heard, which says the agarose solution should be left to cool first, or 
the etbr added in a fume cupboard. Obviously this later method seems to be the 
safest, but is there any merit to their claim?

Methods mailing list
Methods at

This email message may contain legally privileged and/or confidential information.  If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited.  If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

More information about the Methods mailing list