Weak binding of His-tags to IMAC

[Duncan Clark]

Historians believe that in newspost <4330f46c$1 at clarion.carno.net.au> on 
Wed, 21 Sep 2005, Bean Long <ben.long at yourfinger.anu.edu.au> penned the 
following literary masterpiece:
>Any ideas appreciated.

If the HIS tag is buried in your protein and therefore not accessible, 
then nothing you do with buffers will have any affect - it will not 
bind.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.