Weak binding of His-tags to IMAC

Louis Hom lhom at OCF.Berkeley.EDU
Wed Sep 21 10:45:11 EST 2005


Depending on how desperate you are, you might start trying different 
metals on your column (e.g., Cu2+ or Zn2+).  I think Cu is supposed to 
give stronger binding (sometimes too strong?).
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    


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