Weak binding of His-tags to IMAC

Bean Long ben.long at yourfinger.anu.edu.au
Wed Sep 21 19:01:29 EST 2005


Thanks for all the replies.  I can get weak binding both under native and
denatured conditions, so I don't believe that accessibility is a problem.  I
have several other IMAC resins to try and will have a go with them today.

Many thanks,

-- 
Bean

Remove "yourfinger" before replying
"Duncan Clark" <blackhole at abuse.plus.com> wrote in message
news:wGuPSmaNXYMDFABN at abuse.plus.com...
> Historians believe that in newspost <dgrv67$28kv$1 at agate.berkeley.edu>
> on Wed, 21 Sep 2005, Louis Hom <lhom at OCF.Berkeley.EDU> penned the
> following literary masterpiece:
> >Depending on how desperate you are, you might start trying different
> >metals on your column (e.g., Cu2+ or Zn2+).  I think Cu is supposed to
> >give stronger binding (sometimes too strong?).
>
> Using IDA-sepharose (Amersham's Chelating -sepharose) we quite often
> found that Co2+ is better than Ni2+, in terms of binding capacity and
> specificity of elution.
>
> Duncan
> -- 
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.




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