FW: phenol chloroform

Deanne Bell dbell at fresno.ars.usda.gov
Thu Sep 22 15:28:55 EST 2005


 >-----Original Message-----
>hi
>could someone please tell me what's the exact role of phenol 
>chloroform in DNA extraction?
>

Hydrophobic cell components (such as chlorophyll) from the mechanical /
detergent disruption will get separated into the phenol chloroform layer
of the DNA prep and some proteins get denatured, thus isolating the
nucleic acids into the upper aqueous phase of the prep.

HTH
Dee Bell



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