phenol chloroform

Jayakumar, R R.Jayakumar at roswellpark.org
Thu Sep 22 18:59:33 EST 2005


They help to denature and precipitate the proteins in the DNA extract.  It does not affect the nucleic acids.  Just to clear up the muck.  A second wash with chloroform alone helps to remove any traces of phenol in the acqueous extract.  Traces of phenol can still destroy any DNA modification enzymes used later.  Some people add isoamylalcohol too (1 part in 25) to prevent frothing.
   Hope that helps 
jai
 

________________________________

From: methods-bounces at oat.bio.indiana.edu on behalf of parsi.parto at gmail.com
Sent: Thu 9/22/2005 4:01 AM
To: methods at magpie.bio.indiana.edu
Subject: phenol chloroform



hi
could someone please tell me what's the exact role of phenol chloroform
in DNA extraction?

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