phenol chloroform

Jayakumar, R R.Jayakumar at
Thu Sep 22 18:59:33 EST 2005

They help to denature and precipitate the proteins in the DNA extract.  It does not affect the nucleic acids.  Just to clear up the muck.  A second wash with chloroform alone helps to remove any traces of phenol in the acqueous extract.  Traces of phenol can still destroy any DNA modification enzymes used later.  Some people add isoamylalcohol too (1 part in 25) to prevent frothing.
   Hope that helps 


From: methods-bounces at on behalf of parsi.parto at
Sent: Thu 9/22/2005 4:01 AM
To: methods at
Subject: phenol chloroform

could someone please tell me what's the exact role of phenol chloroform
in DNA extraction?

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