ligation headache!!!!

Mark nospam at
Fri Sep 23 08:16:17 EST 2005

Benzo wrote:
> Hi, I'm trying to ligate a 3.8kb fragment into a 8kb vector using T4
> ligase for a month already. but the ligation reaction didn't seem to
> work.... can anyone out there please help me.......
Please tell us more details. What controls have you tried in 
order to work out what is wrong? How have you prepared your 
insert and vector? Are there blunt or cohesive ends. etc etc.


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