PCR Ligation Issues!!!

ChenHA hzhen at freeuk.com
Tue Sep 27 12:19:05 EST 2005


On 27 Sep 2005 09:52:41 -0700, "jssherkow" <jssherkow at gmail.com>
wrote:

>I have, on pg. 258, that 3 bp from the end of a "linearized vector,"
>cleavage proceeds with 90% efficiency. Do you have something
>different?--Jake

Mine is on a different page, but you should read what is written above
the table (it says you should add 4 extra bases just to be sure).  The
table I use is on the opposite page (yours might be on a differnt
page) for double stranded oligonucleotides.






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