transfect human cells with ligation mix

Icejust noemail at
Tue Sep 27 17:41:23 EST 2005

vagos wrote the Tue, 27 Sep 2005 21:18:32 GMT in 
news:1127855912.109673.167800 at

> Hello everyone,
> is there any possible way I can transfect human cells (fibroblasts, or
> Ad293) with my ligation mix (double-digested dephosphorylated vector +
> insert)?

I think you won't have enough DNA to perform directly the transformation 
and have a reasonable yield. You should ligate, transform and do a midi or 

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