secreted eukaryotic HIS-recombinant protein

troels.wind at troels.wind at
Wed Apr 5 03:51:14 EST 2006

If your problem is a chelating agent, you could try running your sample
over a chelex column before applying it to the Ni column.

> Dear Wolfgang.
> Thanks for the suggestion, we will give it a try. It seems like there is
> some chelating factor in our media that we can not get rid of through
> dialysis, so precipitation is a good suggestion.
> A.Salanti.
> DK

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