Schlierens or Swirls in PolyAcrylamide Gradient Gel

Duncan Clark blackhole at abuse.plus.com
Thu Apr 6 04:08:47 EST 2006


Historians believe that in newspost 
<mailman.484.1144271920.16885.methods at net.bio.net> on Wed, 5 Apr 2006, 
Tony Nugent <tnugent at bhlinc.com> penned the following literary 
masterpiece:
>For others who cast gels, do you see these faint schlierens, and have
>you any experience with them.

It sounds like possibly insufficient mixing of the high and low in the 
gradient mixer so you randomly get a slug of low or high acrylamide 
where it shouldn't be?

Can you stick a dye or something in one side and make it more visible to 
see the problem as it happens?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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