5'end non-radioactive labelling

Austin P. So (Hae Jin) nobody at nowhere.com
Fri Apr 7 22:29:26 EST 2006


I think it may help if you could describe what you want to introduce. 
All PNK will do is transfer the gamma phosphate of ATP onto the 5'-OH of 
the ss/ds DNA.

Mind you, people then introduce a label via the 5'-Phosphate, but I have 
yet to see a publication that indicates the expected yields. I think 
Vector sells a kit.

Austin

tania lombo wrote:
> I would like to know if there is any company/method by which I can do 5'end PNK labelling with modified ATP.
>   I will really appreciate any suggestion.
>   Sincerely
>   Tania Lombo


More information about the Methods mailing list