Re-POST of THE ORIGINAL MERLIN MINI/MAXI-PREP

Wolfgang Schechinger novalidaddress at nurfuerspam.de
Mon Apr 10 12:47:20 EST 2006


Kyle,

I store the EtOH in the -20 in order to prevent oxidation to
Acetaldehyde (A) by oxygen and light. Traces of A might react with the
DNA (am I paranoid?).  For the removal of traces of salt etc, you'll be
better off when you work at RT (as you will anyway unless you do all
the prepping in the icehouse or in a refrigerated centrifuge). At least
DNA (in)solubility -as frequently told- should not be the reason why
one stores the EtOH in the freezer.

Probably also should be the same for iPropOH as its oxidation product
aceton also might react with DNA.

To all Bionetters: Please comment! Should one be concerned about it or
doen't it matter at all?

Wolfgang



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