Cloudy TBS solution.

Jayakumar, R R.Jayakumar at roswellpark.org
Mon Apr 10 20:55:00 EST 2006


You don't need to autoclave TBS if you are going to use it only for
westerns, though filtering it is a good habit.   How do you adjust the
pH?? I mean what acid do you use?
 
Jai



-----Original Message-----
From: methods-bounces at oat.bio.indiana.edu
[mailto:methods-bounces at oat.bio.indiana.edu] On Behalf Of Ved Sharma
Sent: Monday, April 10, 2006 3:56 PM
To: methods at magpie.bio.indiana.edu
Subject: Cloudy TBS solution.

Hi,

I'm having trouble keeping 1X TBS solution clear/transparent. It 
becomes cloudy after a couple of days. I make it by diluting 10X 
TBS solution and then adjusting the pH to 7.3. I don't autoclave 
after that. Is that the reason why it gets cloudy?

Thanks!

Ved

----------------------------------
Ved P. Sharma
Graduate Student
Department of Chemical Engg
University of Florida
Gainesville, FL-32611
-----------------------------------

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