Cloudy TBS solution.

Ved Sharma vedp at
Tue Apr 11 00:04:28 EST 2006

Thanks for your reply.

I'm using TBS for immunohistochemistry and I adjust the pH by HCl. 
I never tried filtering it because when I prepare the solution, it 
looks very clear. Can it be a bacterial contamination which 
develops after a couple of days? Although it's hard for me to 
imagine if bacteria can live on a "Tris base-NaCl" diet.


On Mon Apr 10 21:55:00 EDT 2006, "Jayakumar, R" 
<R.Jayakumar at> wrote:

> You don't need to autoclave TBS if you are going to use it only 
> for
> westerns, though filtering it is a good habit.   How do you 
> adjust the
> pH?? I mean what acid do you use?
>  Jai
> -----Original Message-----
> From: methods-bounces at
> [mailto:methods-bounces at] On Behalf Of Ved 
> Sharma
> Sent: Monday, April 10, 2006 3:56 PM
> To: methods at
> Subject: Cloudy TBS solution.
> Hi,
> I'm having trouble keeping 1X TBS solution clear/transparent. It 
> becomes cloudy after a couple of days. I make it by diluting 10X 
> TBS solution and then adjusting the pH to 7.3. I don't autoclave 
> after that. Is that the reason why it gets cloudy?
> Thanks!
> Ved
> ----------------------------------
> Ved P. Sharma
> Graduate Student
> Department of Chemical Engg
> University of Florida
> Gainesville, FL-32611
> -----------------------------------
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