cryopreserved primary cultured mouse cortical neurons

[Bhaswati Banerjee]

Hello,
   
  Has anyone used the commercially available cryopreserved primary cultured mouse cortical neurons for immunocytochemistry or other experiments and thereby optimized the culture conditions? If so please let me know the conditions of coating the coverslips, like what is the final dilution of Poly L lysine / Poly D Lysine used? What is the maximum yield of neurons and what are the culture conditions and anything  relevant would be helpful. A detailed protocol will be highly appreciated as well.
   
  Thanks 
  Bhaswati
   
  Bhaswati Bandyopadhyay
Postdoctoral Researcher
Center for Molecular Neurobiology
1060 Carmack Rd.
Columbus, OH 43210
Tel: (614) 688-5894
Fax: (614) 292-5379
Email: bandyopadhyay.4 at osu.edu
   
   

		
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