slide assay with vista green. It's possible?

GysdeJongh jongh711 at planet.nl
Tue Apr 11 08:20:34 EST 2006


"gberna" <gberna at gmail.com> wrote in message 
news:1144742168.582362.54550 at v46g2000cwv.googlegroups.com...
> Dear,
> I have a question.
> I want to see if in my slides the DNA is locked on surface (polylysina)
> immediately after spotting procedure.
> My Boss said me that there are some product  like "Vista Green" that
> allow to see immediately (with scanner) if the DNA are onto slides, and
> after I can clean the same slide and perform a normal  hybridization
> Someone can help me to understand which product I can use and if it's
> possible?

Hi gberna ,
I  _guess_  from your post that you are working with DNA Microarray's and 
that you are using the sort where DNA oligo's are spotted on poly-lysine 
coated slides. If that is true look here for more information :

http://groups.yahoo.com/group/microarray/

I use a special designed random 9-mer Oligo which has a 5'-Cy5 label.I do 
one or two test hybridisations with this Oligo for each batch of printed 
Microarray's , just following our standard protocol.This will stain all the 
spotted DNA's equally , if the Microarray's are OK

hth
Gys




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