Cloudy TBS solution.

Nick Theodorakis nick_theodorakis at
Tue Apr 11 09:27:13 EST 2006

Ved Sharma wrote:
> Thanks for your reply.
> I'm using TBS for immunohistochemistry and I adjust the pH by HCl.
> I never tried filtering it because when I prepare the solution, it
> looks very clear. Can it be a bacterial contamination which
> develops after a couple of days? Although it's hard for me to
> imagine if bacteria can live on a "Tris base-NaCl" diet.

You'd be surprised at what they grow in.

Does it smell funny when you see the turbidity? It's not always a sure
thing, but if it smells you definitely have contamination.


Nick Theodorakis
nick_theodorakis at
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