About His-tag purification

Zheng.Chou zheng_chou at 126.com
Wed Apr 12 10:33:16 EST 2006

 I'm expressing a HIS-tagged 16KDa protein in E.coli

I got the target protein using the SDS-PAGE analysis

However, the UV absorbance is 260 nm but not 280

I wander wether the protein binding with DNA/RNA in E.coli lysate

How can I improve

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