slide assay with vista green. It's possible?
jongh711 at planet.nl
Thu Apr 13 06:03:32 EST 2006
"gberna" <gberna at gmail.com> wrote in message
news:1144851682.438536.197630 at z34g2000cwc.googlegroups.com...
> Thanks Gys,
> I've seen your link , but I'm finding,if it's possible, a tecnique that
> allow me to look immediatly after spotting if my DNA (PCR) are blocked
> onto slide.
> I need this becouse, I work in a university, but the spotter and the
> scanner are located in a a differt city were I can only Spotting and
> (This is the Italy!!!)
Hi gberna ,
you can see the autofluorescence of the spotted DNA just after spotting and
baking or UV croslinking with a normal scanner . Fast . After washing and
blocking , the autofluorscence of the DNA is nolonger visible. However you
can stain the array , at that point , very quicky with SbrGreen or SybrGold
. Dip , wash with MilliQ and dry . Fast . We use the hybridisation with the
synthetic Oligo because this tests the whole process from begin to end and ,
in our hands , is the most reliable quality control .
Or you could buy Affi- or ABI chips . Faster .
They have another advantage : they are the only one accepted for publication
! You will not get a publication with home brew chips in any serious
There are a number of companies that provide a complete service. You send
them your RNA , they give you a DVD with the gene regulations and the
bioinformatics . Completely interpreted . The fastest :)
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