Promoter isolation

StewJW stewjw at
Mon Apr 24 04:14:08 EST 2006

Invitrogen sells a TOPO® Walker Kit which should help with the PCR:

The TOPO® Walker Kit is specifically designed to fill in the gaps that
develop when sequencing long stretches of DNA. The kit utilizes
Vaccinia virus topoisomerase I to join a 58 bp linker containing primer
binding sites to the unknown end of a desired DNA fragment, allowing
subsequent PCR amplification. The resulting PCR products can be
sequenced directly or cloned for further analysis."
Fisher Code:    VXK800001

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