problems sequencing plasmids prepped from TG1
novalidaddress at nurfuerspam.de
Tue Apr 25 08:41:54 EST 2006
we try to get sequences clones from a phage display experiment grown in
from about 60 clones we had sent to a sequencing firm, only 9 yielded
data. the company said the DNA was in best condition. the plasmids were
prepped with a quiagen miniprep kit.
we had tested several samples by PCR (one of the primers is th e
sequencing primer,no problems, if you can compare PCR and sequencing at
all), and we find a band with the size of the expected insert in
we are quite clueless now.
our next options would be re-transforming the plasmid in DH5alpa and
sending samples to some more labs.
any ideas what could be wrong and what to do?
Endocrine Research Lab
Bochum University Hospital "Bergmannsheil"
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