I need a solution for purification of an his-tagged protein

Bean Long ben.long at yourfinger.anu.edu.au
Tue Apr 25 18:14:34 EST 2006


Bezeille,

Did you use some imidazole in your Ni column binding buffer??  5-20 mM 
imidazole in the binding buffer should help eliminate some of the 
non-specific binding.

-- 
Bean

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