protein activity with extra N-terminal peptide

Isaac Li isaac.li at utoronto.ca
Fri Apr 28 15:53:52 EST 2006


Hi,
I have a expression vector based on the pTriX1.0 vector.
the vector was constructed with three cut sites (Nco, Spe, BamH) in 
front of the protein to be expressed, which results in expression with 
the few extra residues before the real start codon of the protein:
M G L T S G S - [M ... actualy protein sequence]
would this be a problem to affect the activities of the protein i am 
expressing?
do general expressing vectors have extra residues before the actual protein?
Thanks very much
Isaac


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