protein activity with extra N-terminal peptide
Trond Erik Vee Aune
trondaun at nt.ntnu.no
Sat Apr 29 02:32:09 EST 2006
Isaac Li wrote:
> I have a expression vector based on the pTriX1.0 vector.
> the vector was constructed with three cut sites (Nco, Spe, BamH) in
> front of the protein to be expressed, which results in expression with
> the few extra residues before the real start codon of the protein:
> M G L T S G S - [M ... actualy protein sequence]
> would this be a problem to affect the activities of the protein i am
It really depends on the protein and it's overall function/structure.
Some proteins have no problem coping with a few extra residues, others
would not function properly.
> do general expressing vectors have extra residues before the actual
Generally one aims to clone the gene of interest into the first ATG
downstream of the translation initiation region.
That said, many commercial vectors contain different N-terminal fusion
partners to achieve effecient translocation, expression, isolation, etc.
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