PCR on cells to identify transfectants?

Markus Winter beyonder at blueyonder.co.uk
Thu Aug 3 15:16:13 EST 2006


Hi all,

I have a lot of transfectant clones to test - which usually means
isolating RNA, making cDNA, and then running a PCR for the transgene.

But is there a quick way to test whether the whole expression cassette
is intact by PCR? And then just isolate RNA from those clones?

Thanks for any advice you can give

Markus



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