Double stable transfection

Allison allison at
Mon Aug 7 09:21:19 EST 2006

sylvain31 wrote:
> Hello,
> I would like to establish a double stable transfection of CHO cells
> with two partners but in a single transfection step. The goal of this
> strategy is to avoid two consecutive single stable transfections which
> is time consumming. Thus, does somebody know if expression vectors able
> to contain and promote expression of both transgenes are commercially
> available. If no, alternative strategies proposed will be welcomes.
> Thanks a lot.

There is the pBUDCE4.1 vector from Invitrogen for expressing two 
transcripts at the same time.  Have bought it but haven't tried it yet.

More info at


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