Double stable transfection

Allison allison at nospam.com
Mon Aug 7 09:21:19 EST 2006


sylvain31 wrote:
> Hello,
> I would like to establish a double stable transfection of CHO cells
> with two partners but in a single transfection step. The goal of this
> strategy is to avoid two consecutive single stable transfections which
> is time consumming. Thus, does somebody know if expression vectors able
> to contain and promote expression of both transgenes are commercially
> available. If no, alternative strategies proposed will be welcomes.
> Thanks a lot.
> 

There is the pBUDCE4.1 vector from Invitrogen for expressing two 
transcripts at the same time.  Have bought it but haven't tried it yet.

More info at http://tinyurl.com/zofhj

HTH
Allison


More information about the Methods mailing list