lipase, & cellulase test

StewJW stewjw at gmail.com
Mon Aug 14 03:44:25 EST 2006


Invitrogen privides a fluorescent substrate for the quantification of
Cellulase (Ex/Em 360/460 nm, detection limit of 40 µU/ml). If you
don't have a flourometer you can also measure the increase in
absorption at 360 nm; see manual below:

http://probes.invitrogen.com/media/pis/mp33953.pdf


Invitrogen also offers a triacylglycerol-based lipase substrate
(E33955)  In the presence of lipases, the nonfluorescent EnzChek lipase
substrate produces a bright, green-fluorescent product
(excitation/emission maxima of ~505/515 nm) for the detection of lipase
activity in solution.



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