Double stable transfection

Brian Ballard NoReply at email.com
Mon Aug 14 14:36:38 EST 2006


In article <mailman.590.1155574139.20007.methods at net.bio.net>,
 <arnaud.garcon at syngenta.com> wrote:

>  
> I may have not expressed myself correctly. The conditions you need to
> scout with two plasmids co-transfection are the transfection conditions
> (ie amount of DNA/liposome etc). If you're expressing a protein that
> requires the assembly of 2 subunits (eg. an antibody) then the
> respective expression level of each can be quite critical for the
> overall expression. But you're right the antibiotics are used at
> standard kill-curve concentration.
> I found the crux in this type of experiment was to screen a sufficent
> number of cell lines to get a good level of expression.
> 
> A.-

Hi Arnaud - that makes a lot more sense!

Brian


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