Plasmids for the production of 100bp and 1kb DNA ladders

Tom Knight tk at mit.edu
Fri Aug 25 08:39:48 EST 2006


"news.uni-muenster.de" <seggewis at gmx.de> writes:
> I search for suitable plasmids for the production of 100bp and 1 kb DNA 
> ladders, respectively.
> Does anybody have these plasmids who would send them?

It is far easier to order a dozen oligos and PCR the ladder fragments
from a handy backbone (I use pUC19).  You get to control the amounts
of each fragment this way -- important for the small ones.




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