Plasmids for the production of 100bp and 1kb DNA ladders

Christian Praetorius prae at gmx.net
Fri Aug 25 09:08:25 EST 2006


Tom Knight <tk at mit.edu> wrote:

>It is far easier to order a dozen oligos and PCR the ladder fragments
>from a handy backbone (I use pUC19).  You get to control the amounts
>of each fragment this way -- important for the small ones.

Right. But is this really cheaper and easier than buying ready-to-use
markers?

Christian

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