Plasmids for the production of 100bp and 1kb DNA ladders

ChenHA hzhen at freeuk.com
Fri Aug 25 09:40:45 EST 2006


On Fri, 25 Aug 2006 14:49:51 +0200, "news.uni-muenster.de"
<seggewis at gmx.de> wrote:

>Hello!
>
>I search for suitable plasmids for the production of 100bp and 1 kb DNA 
>ladders, respectively.
>Does anybody have these plasmids who would send them?
>I know that I could use Lambda/BstI, Lambda/HindIII and so on. However, I 
>would like to use my own 100bp and 1kb DNA ladder.
>
>Or does anybody have a suitable, tested protocoll with plasmids, sequences 
>and restriction enzymes so that I could construct the pülasmids on my own?
>
>Thank you for your help!
>
>Jo 
>

I don't have the plasmid here, but you can make them yourself if you
want to.   The reference for this is 

Anonymous (1989) BRL Focus 11,36

Unfortunately I don't have the paper as well, and it is no longer
available on the web, when Gibco-BRL disappeared into Invitrogen.  So
my question  is - has anyone a copy of this or know where I can find
one?






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