uneven running pattern in agarose gel

ali atahery at gmail.com
Mon Aug 28 13:54:58 EST 2006

When I run the PCR reactions in 1% agarose gel (big gels 350ml at
115V), sometimes it gives me uneven pattern that is not due to the
polymorphism and it is clear that there is a problem with the gel or
running buffer or the Machine. What could be the main cause?

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