protein sticking to membranes

Wolfgang Schechinger novalidaddress at nurfuerspam.de
Tue Aug 29 15:03:38 EST 2006


Dear Ho,

seems you protein likes polar surfaces a lot. Made me instantly think
of non-stick frying pans an thus I would like to recommend teflon
membranes.

Wolfgang

Ho-Leung Ng wrote:
> Hello,
>
>      I am working with a protein that sticks to membranes used in dialysis
> and concentrators. I've tried both PES and cellulose membranes. This
> makes buffer exchange and concentrating a huge pain. The protein is
> well behaved in solution, and the stickiness is not due to
> aggregation. I can recover some of my protein by washing the membrane
> afterward with buffer, but the yield is not great and inconsistent.
> Are there other membranes worth trying? Does anyone else have
> experience with sticky proteins?
> 
> 
> Thank you,
> ho



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