Separation of a truncated protein by ion exchange chromatography

Alex alexanderaleshin at yahoo.com
Tue Aug 29 19:45:37 EST 2006


My protein expresses as two species with predicted pI=9.3 and pI=8.8
due to truncation of 14 N-terminal residues (out of 400). I've tried to
separate them by Mono-S column in a buffer with pH=7.5, but failed.

QUESTION: Is it possible to separate such proteins by the ion exchange
chromatography? Any suggestion about the buffer, pH, or other
techniques excluding preparative isoelectrofocusing?



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