Double stable transfection

StewJW stewjw at gmail.com
Wed Aug 30 08:51:03 EST 2006


Invitrogen has a cell line database which has useful refs  and
protocols in its Support section.

For INS it lists:
1. Source: J Biol Chem 1997 272(3)1929-34
Comments: INS-1 cells were transiently transfected with plasmid DNA
using Lipofectamine.
Scientific Application(s): Transient Transfection
Product Name and Cat No: Lipofectamine Transfection Reagent (18324111)

 2. Source: Nucleic Acids Res 2004 32(19):e150-e150
Comments: INS-1 cells were stably transfected with plasmid DNA using
lipofectamine...
Scientific Application(s): Stable Cell Transfection
Product Name and Cat No: Lipofectamine Transfection Reagent (18324111)


I'd consider using the newer Lipofectamine LTX which is less toxic.



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